Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
Pongrácz Judit Erzsébet
professor,
Department of Pharmaceutical Biotechnology
Number of hours/semester
Lectures: 28 hours
Practices: 0 hours
Seminars: 0 hours
Total of: 28 hours
Subject data
- Code of subject: OBH-008-E
- 2 Credit
- Biotechnology MSc
- Specialty in Medical Biotechnology module
- autumn
haven't
Course headcount limitations
min. 1 people – max. 50 people
Topic
The course material is designed to provide theoretical background to currently employed three dimensional tissue culture techniques including scaffold-based (biodegradable) and scaffold-free (tissue printing, pelleting and hanging-drop) systems. The course will detail the importance of the tissue microenvironment in normal and artificial tissue development. Directed cellular growth, organ formation for surgical implantation and drug target identification in human tissue systems and in vitro testing of pharmaceutical compounds in complex tissue systems are also part of the curriculum. The course will discuss the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions.
Lectures
- 1. Introduction, basic principles of tissue engineering (TE) - Bóvári-Biri Judit
- 2. 2D and 3D cell and tissue culture - Bóvári-Biri Judit
- 3. Cells and tissue types in tissue engineering I: stem cell types, their characteristics and potential applications (hESCs, iPSCs, fetal stem cells) - Bóvári-Biri Judit
- 4. Cells and tissue types in tissue engineering II: stem cell types, their characteristics and potential applications (adult somatic stem cells, eg. ADSCs, dental stem cells). Differentiated, organ-specific primary cells. - Bóvári-Biri Judit
- 5. Bioreactors, requirements of bioreactor design. Characteristics and use of the main bioreactor types I. - Bóvári-Biri Judit
- 6. Characteristics and use of the main bioreactor types II. - Bóvári-Biri Judit
- 7. Scaffolds in tissue engineering. Types and characteristics of frequently used biomaterials I. - Bóvári-Biri Judit
- 8. Types and characteristics of frequently used biomaterials II. - Bóvári-Biri Judit
- 9. Degradation of polymer scaffolds. Technologies of scaffold fabrication I. - Bóvári-Biri Judit
- 10. Technologies of scaffold fabrication II. - Bóvári-Biri Judit
- 11. Cell-scaffold interactions. Scaffold parameters affecting cell behavior. - Bóvári-Biri Judit
- 12. Biofactors in TE. Controlled release of bioactive factors in engineered tissues. Drug delivery systems in TE I. - Bóvári-Biri Judit
- 13. Drug delivery systems in TE II. Biosensors in TE. - Bóvári-Biri Judit
- 14. Types and use of aggregate cultures. Organoids and spheroid cultures. - Bóvári-Biri Judit
- 15. Technologies used for the generation of aggregate cultures I. - Bóvári-Biri Judit
- 16. Technologies used for the generation of aggregate cultures II. Microfluidic systems. - Bóvári-Biri Judit
- 17. Advanced tissue models using transwell inserts: Air-Liquid Interface (ALI) technology and Blood-Brain-Barrier (BBB) models. - Bóvári-Biri Judit
- 18. Test - Bóvári-Biri Judit
- 19. Tumor models I. - Bóvári-Biri Judit
- 20. Tumor models II. - Bóvári-Biri Judit
- 21. Tissue printing technologies I. - Bóvári-Biri Judit
- 22. Tissue printing technologies II. - Bóvári-Biri Judit
- 23. Tissue engineering for transplantation I. Timeline and milestones of organ and tissue transplantation - Bóvári-Biri Judit
- 24. Hematopoietic stem cell transplantation. - Bóvári-Biri Judit
- 25. Tissue engineering for transplantation I. TE of pancreatic islets - Bóvári-Biri Judit
- 26. Tissue engineering for transplantation II. Cartilage TE. - Bóvári-Biri Judit
- 27. Tissue engineering for transplantation II. Skin substitutes. - Bóvári-Biri Judit
- 28. Types and regulation of advanced therapy medicinal products (ATMPs). - Bóvári-Biri Judit
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Lecture ppt
Notes
Three dimensional tissue cultures and tissue engineering -access via www.gytk.hu
Three dimensional tissue cultures and tissue engineering - access via www.tankonyvtar.hu
Recommended literature
Pongracz & Keen: Medical Biotechnology, Elsevier, 2009
Joseph D. Bronzino: Tissue Engineering and Artificial Organs
Ali Khademhosseini (ed.): Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications (Engineering in Medicine & Biology)
Challa S. S. R. Kumar (ed.): Tissue, Cell and Organ Engineering (Nanotechnologies for the Life Sciences)
W. Mark Saltzman: Tissue Engineering: Engineering Principles for the Design of Replacement Organs and Tissues
Robert Lanza, Robert Langer, Joseph Vacanti: Principles of Tissue Engineering, 3rd edition
Will W. Minuth, Raimund Strehl, Karl Schumacher: Tissue Engineering: From Cell Biology to Artificial Organs
Fen Ontaigne: Medicine by Design: The Practice and Promise of Biomedical Engineering, Forgacs & Newman, 2005.
Conditions for acceptance of the semester
75% attendance to lectures and completion of the midterm test, then final exam
Mid-term exams
Midterm test
Making up for missed classes
None
Exam topics/questions
Written test. Online Unipol system, Teams, Moodle
Examiners
- Bóvári-Biri Judit
Instructor / tutor of practices and seminars
- Bóvári-Biri Judit
- Steinerbrunnerné Nagy Alexandra