Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
Perjési Pál
professor,
Department of Pharmaceutical Chemistry
Number of hours/semester
Lectures: 28 hours
Practices: 0 hours
Seminars: 0 hours
Total of: 28 hours
Subject data
- Code of subject: OTF-SEP-T
- 2 Credit
- Biotechnology BSc
- Optional module
- spring
OTN-ANLC-T finished , OTN-GENC-T finished , OTN-PHYC-T finished
Exam course:Course headcount limitations
min. 5 people – max. 20 people
Topic
Within the frame of this subject, students will study the most commonly used (chromatographic) separation methods of small molecules and macromolecules . This knowledge is crucial for almost all fields of the pharmaceutical, food, and environmental industries.
Lectures
- 1.
Short history of chromatography. Classification of chromatographic methods
- Perjési Pál - 2.
Short history of chromatography. Classification of chromatographic methods
- Perjési Pál - 3.
Theoretical bases of chromatographic separation.
- Perjési Pál - 4.
Theoretical bases of chromatographic separation.
- Perjési Pál - 5.
Column chromatograpy I. Adsorptiion column chromatography
- Perjési Pál - 6.
Column chromatograpy I. Adsorptiion column chromatography
- Perjési Pál - 7.
Column chromatography II. Partitition chramatography. Modification of the surface of adsorbents.
- Perjési Pál - 8.
Column chromatography II. Partitition chramatography. Modification of the surface of adsorbents.
- Perjési Pál - 9.
Column chromatography III. Methods of detection
- Perjési Pál - 10.
Column chromatography III. Methods of detection
- Perjési Pál - 11.
Ion exchange chromatography. Test Paper 1
- Perjési Pál - 12.
Ion exchange chromatography. Test Paper 1
- Perjési Pál - 13.
Gel chromatography
- Perjési Pál - 14.
Gel chromatography
- Perjési Pál - 15.
Affinity chromatography. Immobilization methods.
- Perjési Pál - 16.
Affinity chromatography. Immobilization methods.
- Perjési Pál - 17.
Thin layer chromatography
- Perjési Pál - 18.
Thin layer chromatography
- Perjési Pál - 19.
High pressure liquid chromatography
- Perjési Pál - 20.
High pressure liquid chromatography
- Perjési Pál - 21.
Gas chromatography. Methods of detection
- Perjési Pál - 22.
Gas chromatography. Methods of detection
- Perjési Pál - 23.
Supercritical chromatography. Test Paper 2
- Perjési Pál - 24.
Supercritical chromatography. Test Paper 2
- Perjési Pál - 25.
Electromigration methods
- Perjési Pál - 26.
Electromigration methods
- Perjési Pál - 27.
Liquid-liquid extraction, Solid-phase extraction
- Perjési Pál - 28.
Liquid-liquid extraction, Solid-phase extraction
- Perjési Pál
Practices
Seminars
Reading material
Obligatory literature
N/A
Literature developed by the Department
Powerpoint presentations.
Notes
N/A
Recommended literature
N/A
Conditions for acceptance of the semester
Acknowledgment of the course is in accordance with the Code of Studies and Examinations. Participation in both the lectures and the course practices is obligatory. A maximum of three absences from lectures are allowed.
Mid-term exams
Two compulsory midterm tests will be written during the semester on the 6th and the 12th weeks. One of the test results should be above 60%, and the average should be above 50%. One retake test is allowed on the 13th week of the semester. The result of the retake test is added to the previous two.
Making up for missed classes
There is no possibility of making up missed classes.
Exam topics/questions
Two semester tests.
Examiners
- Perjési Pál